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. 2015 Jan 15;14(6):838–847. doi: 10.1080/15384101.2014.1000212

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Figure 1. — Vifs that are competent for APOBEC3H removal cannot induce cell cycle arrest. (A) Representative flow cytometry data showing Vif_NL4–3's or Vif_HXB2's potency in cell cycle regulation in HEK293T cells. (B) Bar chart representing the (G2/M)/G1 ratio of HEK293T cells transfected with Vif_NL4–3 or Vif_HXB2. Western blotting results (above) indicate the protein expression levels of Vif_NL4–3 and Vif_HXB2. (C) Western blotting results for APOBEC3H levels in the presence of Vif_HXB2 or Vif_NL4–3 in HEK293T cells. (D) Western blotting results for APOBEC3G levels in the presence of Vif_HXB2 or Vif_NL4–3 in HEK293T cells. (E) Western blotting results for APOBEC3F levels in the presence of Vif_HXB2 or Vif_NL4–3 in HEK293T cells. (F) Bar chart representing the (G2/M)/G1 ratio of HEK293T cells transfected with different Vifs that are potent in depleting APOBEC3H, with the exclusion of BNL43. (G) Western blotting results indicating the protein expression levels of the Vif proteins in (F).