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G2-related positions are also critical for Vif's induction of APOBEC3H depletion. (A) VifHXB2 and VifNL4–3 mutants used in this figure. (B) Western blotting results for APOBEC3H levels in the presence of VifHXB2 and its mutants at G2-related positions. VifNL4–3 was introduced as a negative control. (C) Western blotting results for APOBEC3H levels in the presence of VifHXB2, VifNL4–3, and their M5 mutants (with positions 31, 33, 36, 47, and 50 exchanged between VifHXB2 and VifNL4–3).
