Figure 6.

The Vif protein uses similar regions to induce G2 arrest and the degradation of APOBEC3H. (A) Surface model of the Vif-CBFβ interaction based on the published structure (PDB: 4N9F).¹⁹ The Vif protein is labeled in red, and CBFβ is in yellow. On the Vif protein, the APOBEC3F-binding region is labeled in blue, the APOBEC3G-binding region in wheat, the critical residues for G2 arrest in cyan, and the critical residues for APBOEC3H depletion in green. (B) Detailed conformation of Vif around positions 31, 33, 36 (essential for G2 arrest), and 39 (essential for APOBEC3H depletion). (C) Detailed conformation of Vif around positions 47, 50 (for G2 arrest), and 48 (for APBOEC3H depletion). It is noteworthy that N48 on Vif may interact with F69 and W73 (in purple) on CBFβ.