Figure 5. CaeA treated asthma mice exhibit downregulation of Th2 response.

Allergic asthma induced mice were treated with CaeA1 (1 mg/kg bwt) and CaeA10 (10 mg/kg bwt). Animals suffering from asthma receiving Dex10 (10 mg/kg bwt) were kept as a positive control. A placebo (PBS) administered control groups were also kept of either normal mice or suffering from asthma. The animals were sacrificed and Th2 response was monitored. (A,B) Bar diagram depicts the reduced levels of IL-5 and IL-13 by ELISA in the BALF; (C,D) RT-qPCR data support the decreased expression of IL-5 and IL-13 in lungs; (E) dot plots represent decline in total IgE in BALF; each dot denotes a single mouse; (F) antigen specific IgE content in BALF; (G–I) ELISA data show diminished production of IL-4, IL-5 and IL-13 by lymphocytes in the SNs on in vitro challenged with OVA. Error bar represents mean ± SEM. The results shown are from 2–3 independent experiments and each bar represents means ± SEM of 6 mice per group. nd: not detected. *p < 0.05, **p < 0.005, ***p < 0.0001.