Figure 6.
ON BC signaling is restored following AAV treatment in Nyxnob mice. A representative rod BC expressing AAV-mediated YFP_NYX and properly localized TRPM1 was targeted for whole-cell patch-clamp recording and filled with sulforhodamine B (A). Capsaicin (10 μM), a TRPM1 agonist, was puffed onto the rod BC dendrites to gate the opening of the TRPM1 channel. Vhold = 50 mV. Representative current recordings from WT rod BC, AAV-treated and untreated rod BCs from Nyxnob mice reveal full restoration of ON BC signaling following treatment (B). Capsaicin was puffed onto rod BC dendrites for 200 ms and 1 s durations. Current responses were recorded from each cell and then averaged (C).