Table 1.
Primers used in PCRs of this study
| Gene | Exon | Forward primer (5′→3′) | Reverse primer (5′→3′) | Amplicon size, bp |
|---|---|---|---|---|
| BRAF | 15 | TGCTTGCTCTGATAGGAAAATGA | CCACAAAATGGATCCAGACAAC | 173 |
| CTNNB1 | 3 | ATGGAACCAGACAGAAAAGCGGC | GCTACTTGTTCTTGAGTGAAG | 200 |
| ERBB2 | 20 | CTCTCAGCGTACCCTTGTCC | AGGTGCATACCTTGGCAATC | 220 |
| FOXL2 | 1 | AGAAGGGCTGGCAAAATAGC | GCCGGTAGTTGCCCTTCT | 150 |
| KRAS | 2 | TAAGGCCTGCTGAAAATGACTG | TGGTCCTGCACCAGTAATATGC | 167 |
| PIK3CA | 1 | TGCTTTGGGACAACCATACATC | CTTGCTTCTTTAAATAGTTCATGCTTT | 232 |
| PIK3CA | 1 | CCCCTCCATCAACTTCTTCAA | ATTGTATCATACCAATTTCTCGATTG | 196 |
| PIK3CA | 9 | TTTTCTGTAAATCATCTGTGAATCC | TCTCCATTTTAGCACTTACCTGTGA | 223 |
| PIK3CA | 20 | ATCATTTGCTCCAAACTGACCA | TTGTGTGGAAGATCCAATCCAT | 291 |
| PPP2R1A | 5 | TACTTCCGGAACCTGTGCTC | CCAGGAAGCAAAACTCACCT | 165 |
| PPP2R1A | 6 | GTTCCTGCCCATGAAAGAGA | TTATTGCTCAAACGCCCAAT | 353 |