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. 2015 Jan 20;14(7):1001–1009. doi: 10.1080/15384101.2015.1007001

Figure 2.

Figure 2.

RecQL4 is required for chromatin binding of Mcm10 and Ctf4. (A) HeLa cells were pre-treated with PBS containing 0.1% Triton X-100 for 30 sec, fixed with 3.7% paraformaldehyde, and stained with antibodies against indicated proteins. (B–D) HeLa cells transfected with siRNAs against GL2 or RecQL4” were pre-treated, fixed, and stained with antibodies against indicated proteins (B, D), or fixed without pre-treatment and stained with indicated antibodies (C). Representative examples are shown in (B) and (C), and the percentage of cells showing positive staining of Mcm10 or Ctf4 from the pre-treated cells stained with Cyclin E is presented as bar-graph at (D). Error bars represent the standard deviation from 3 independent experiments. Scale bars represent 10 μm.