Skip to main content
. 2015 Jan 20;14(7):1001–1009. doi: 10.1080/15384101.2015.1007001

Figure 3.

Figure 3.

Interactions between RecQL4, Mcm10, and Ctf4 require the S phase promoting kinases, CDK and DDK. (A) The 293T cells stably expressing FLAG-RecQL4 (left panel) or FLAG-Ctf4 (right panel) were treated with 10 μM NU6140 (NU) or mock-treated (M) for 24 hr, and immunoprecipitation was carried out using anti-FLAG M2 agarose beads. For control immunoprecipitation (lane C), FLAG peptides (0.2 mg/ml) were added to cell extracts for immunoprecipitation. (B) HeLa cells transfected with GL2 (G2) or Cdc7 (C7) siRNAs were incubated for 24 hr, and immunoprecipitations were carried out using antibodies against RecQL4 or Ctf4. (C) Sf9 cells infected with baculoviruses expressing indicated proteins were incubated for 48 hr. Whole cell extracts were prepared and incubated in the absence (lane M), the presence of λ phosphatase (lane λ) or λ phosphatase and sodium vanadate (lane λ + V). Immunoprecipitations were carried out using antibodies against indicated proteins. R4, M10, and C4 denote RecQL4, Mcm10, and Ctf4, respectively.