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. 2015 Jan 20;14(7):1001–1009. doi: 10.1080/15384101.2015.1007001

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Figure 4. — The influence of CDK, DDK and pre-RC proteins on the binding of RecQL4, Mcm10 and Ctf4 to chromatin. (A) HeLa cells were treated with 10 μM roscovitine (ROS) or transfected with siRNA against Cdc7. After incubation for 24 hr, soluble (SF) and chromatin (CF) fractions were prepared, and subjected to Western blotting. GL2 siRNA was used as the negative control. (B) HeLa cells transfected with siRNAs against pre-RC proteins or GL2 were incubated for 24 hr, and the level of indicated proteins in soluble (SF) and chromatin fractions (CF) determined by Western blotting.