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. 2015 Jan 20;14(7):1001–1009. doi: 10.1080/15384101.2015.1007001

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Figure 5. — The association of RecQL4, Mcm10 and Ctf4 with replication origins requires CDK, DDK and pre-RC proteins. (A and B) HeLa cells were transfected with siRNAs against GL2 (G2), Cdc6 (C6), Mcm7 (M7), RecQL4 (R4), Mcm10 (M10) or Ctf4 (C4). After incubation for 24 hr, the binding of RecQL4, Mcm10 or Ctf4 to the replication origin located in the Mcm4 upstream promoter region (M4 Ori) was determined by ChIP analyses. The primer set for the 4 kb distal region (Distal) from the replication origin was used as the internal control in PCR analyses. (C) HeLa cells were transfected with siRNA against Cdc7 (C7) or treated with NU6140 (NU), and ChIP analyses carried out as above.