FIGURE 7.

The effect of nuciferine or siRNA PASK on cell lipid peroxidation in HepG₂ cells of OA-induced hepatic steatosis. (A) GSH. (B) T-AOC. (C) SOD. (D) MDA. SiRNA PASK HepG₂ cells and HepG₂ cells incubated with increased concentrations of nuciferine (NF: 10, 25, and 50 μM) or vitamin E (25 μM) both treated with OA (40 μM). The measurement was described in the Section “Materials and Methods”. Values are Mean ± SEM of three independent experiments performed in triplicates. Significant differences with OA group were designated as ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001. (Control, siRNA scrambled; NF, Nuciferine.)