FIGURE 8.

The effect of nuciferine or siRNA PASK on inflammatory cytokines in HepG₂ cells of OA-induced hepatic steatosis. (A) TNF-α. (B) IL-6. (C) IL-8. (D) IL-10. SiRNA PASK HepG₂ cells and HepG₂ cells incubated with increased concentrations of nuciferine (NF: 10, 25, and 50 μM) or vitamin E (25 μM) both treated with OA (40 μM). The measurement was described in the Section “Materials and Methods”. Values are Mean ± SEM of three independent experiments performed in triplicates. Significant differences with OA group were designated as ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001. (Control, siRNA scrambled; NF, Nuciferine.)