FIGURE 9.

(A) The effect of nuciferine or siRNA PASK on the mRNA level related to inflammation in HepG₂ cells. (B,C) The effect of nuciferine and siRNA PASK on the expression of proteins related to inflammation in HepG₂ cells. SiRNA PASK HepG₂ cells and HepG₂ cells incubated with increased concentrations of nuciferine (NF: 10, 25, and 50 μM) both treated with OA (40 μM). The measurement was described in the Section “Materials and Methods”. Values are Mean ± SEM of three independent experiments performed in triplicates. Significant differences with OA group were designated as those ^∗P < 0.05 and ^∗∗∗P < 0.001. (Control, siRNA scrambled; NF, Nuciferine.)