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Hepatic p-S6 and p-Akt in old and adult (middle-aged) mice. (A) Immunoblot analysis. Protein lysates from the liver of 28 and 10 month old (old vs middle aged) female C57BL/6NCr mice were prepared as described.56,57 Fasted: mice were fasted overnight. Non-fasted: regular conditions. Numbers indicate individual mice. (B) Quantitative analysis of p-S6 signal shown in panel A. Data presented as mean ± SE.
