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. 2014 Oct 30;13(18):2853–2858. doi: 10.4161/15384101.2014.946807

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© 2014 The Author(s). Published with license by Taylor & Francis Group, LLC

© Yuko Murakami-Tonami, Hokuto Ohtsuka, Hirofumi Aiba, and Hiroshi Murakami

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Expression of wee1⁺ and SPCC18B5.02c during meiosis and the effects of mei4⁺ deletion or overexpression. Cells of the indicated genotypes (wild-type (WT), HM1307; mei4, HM2163; mei4⁺-OP, HM4582; WT wee1⁺-HA, HM4732; wee1⁺-HA, mei4, HM4833; and mei4⁺-OP wee1⁺-HA, HM4735) were subjected to synchronous induction of meiosis as described in the Materials and Methods. Samples were collected at the indicated time points thereafter and subjected to Northern blot analysis of wee1⁺ and SPCC18B5.02c mRNA (rRNA (rRNA) was stained with ethidium bromide as the loading control) (A) or Western blot analysis with anti-HA and anti-α-tubulin (loading control) antibodies (B).