Figure 4.
ZO-3 protein expression relies on the presence of ZO-1 in mCCDcl1 cells. (A and B) Cells were transfected 1 day after seeding with siRNA (sc) or siRNA against ZO-1 (siZO-1), ZO-2 (siZO-2) or ZO-3 (siZO-3) and mRNA (A) and protein (B) were isolated 1 - 6 d post-transfection (T1 - T6). Data is represented as fold difference of mRNA expression over values obtained in cells transfected with scrambled siRNA at T1 (for mRNA analysis) or at T2 (for protein analysis) and is expressed as the mean ± SEM of 4 independent experiments. Significant change of expression (P ≤ 0.05) within a same experimental group is depicted by an asterisk. (C) Confocal z-stacks of ZO-1, ZO-2 and ZO-3 depicting their expression in cells transfected with either scrambled siRNA, siZO-1, siZO-2 or siZO-3 at T3. One of 3 similar experiments is shown. Bar, 10 μm.