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. 2015 Oct 20;5:15366. doi: 10.1038/srep15366

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Fully grown oocytes microinjected with Sirt6-MO were cultured in medium with milrinone for 20 hours to repress mRNA translation and then matured in vitro. A sham MO was injected as control. (A) The efficiency of Sirt6-MO was verified by Western blot. Band intensity was calculated using Image J software, and the ratio of Sirt6/actin expression was normalized. (B,C) Quantitative analysis of GVBD and Pb1 extrusion in control (n = 130) and Sirt6-MO (n = 125) oocytes. Bars represent means ± SD of results obtained in 3 independent experiments. *p <0 .05 vs. controls.