Figure 2.

Ultrastructure of the O. dioica germline. (A) Day 5 male mitotic germline nuclei (MiN) are each associated with an MTOC that possesses a pair of centrioles (Ce). (B) Centrioles were not observed within female P3 ovaries. Meiotic nuclei (MeN) possessed nuclear membranes containing nuclear pore complexes (NPC). Golgi apparati were observed in proximity to the nuclear membrane. An electron dense structure of similar size to meiotic nuclei was observed in contact with the nuclear envelope, consistent with the immunofluorescent staining (Fig. 3) of acentriolar OCs. NPCs were observed at the OC/MN interface. (C) OCs were surrounded by endoplasmatic reticulum (ER). M, mitochondria; MVE, multi-vesicular endosome-like structure. (D) Electron dense regions resembling 3 parallel lines, with 100 nm gaps between the central and flanking lines, were observed, consistent with the structure of synaptonemal complexes (SCs). Scale bars in μm.