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. 2015 Aug 15;8(8):12239–12251.

Figure 3.

Figure 3

TGF-β2 induced endothelial mesenchymal transition of HDLECs with increased permeability and weakened recruitment of SMCs. A. Immunofluorescence showed decreased VE-cadherin and increased α-SMA in HDLECs after TGF-β2 treatment (20 ng/ml for 48 h). B. Real time PCR assays revealed decreased VE-cadherin expression and increased α-SMA and SM22α expression. C. Western blots showed the decreased VE-cadherin and increased α-SMA in HDLECs with TGF-β2 treatment. D. Increased permeability was observed in HDLECs after TGF-β2 treatment. E. Decreased PDGF-BB expression in HDLECs after TGF-β2 treatment. F. HDLECs treated with TGF-β2 showed weakened ability to recruit SMCs. All data are presented as mean ± SE from three different experiments with duplicate. *, P < 0.05; **, P < 0.01 versus control group.