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. 2015 Aug 15;8(8):12476–12486.

Figure 1.

Figure 1

Effect of ASO CLU on cell viability and apoptosis of MIA-PaCa-2 cells. A. Western blot analysis of CLU, NF-kB and bcl-2 in whole cell lysates or nuclear of MIA-PaCa-2 cells after treatment with various concentrations (50-1000 nM) of ASO CLU for 48 h. Down-regulation of CLU, NF-kB and bcl-2 expression levels is ASO CLU dose-dependant. β-actin protein as loading control is shown for each blot. 1000 nM ASO CLU could almost completely inhibit the CLU, NF-kB and bcl-2 expression. B. NF-kB DNA binding activity in the nuclear extract of the investigated MIA-PaCa-2 cells in the presence of various concentrations (50-1000 nM) of ASO CLU for 48 h. ASO CLU down-regulated the NF-Kb activity in MIA-PaCa-2 cells. C. Knockdown of CLU failed to induce any significant changes in cell apoptisis of MIA-PaCa-2 cells. D. Knockdown of CLU failed to induce any significant changes in cell viability of MIA-PaCa-2 cells.