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. 2015 Aug 15;8(8):12476–12486.

Figure 4.

Figure 4

ASO CLU influenced sensitive to gemcitabine via NF-kB/bcl-2 dependant pathway. A. ELISA analysis of apoptosis in MIAPaCa-2 cells after treatment with ASO CLU + gemcitabine, ASO CLU + gemcitabine + TNF-α and ASO CLU + gemcitabine + TNF-α + TW-37. Restoration of the NF-kB activity by TNF-α treatment induced a significant decrease in apoptosic cells, and re-inhibition of the bcl-2 expression by TW-37 inTNF-α treated MIAPaCa-2 cells induced a significant increase in apoptosic cells. B. MTT analysis of viable cells, which has the similar results as above. C. Western blot analysis of CLU, NF-kB and bcl-2 in whole cell lysates or nuclear of MIA-PaCa-2 cells above. D. EMSA analysis of NF-kB DNA binding activity in the nuclear extract of the investigated MIA-PaCa-2 cells above.