Effect of APS on the mRNA and protein expression of notch1 and notch3. The H460 cells were incubated with APS (0, 5 or 10 mg/mL) for 48 h, and the cells exposed to APS (5 mg/mL) for 0, 24, 48 and 72 h, the expression levels of notch1 and notch3 were determined by RT-PCR (A) and western blotting (C). mRNA (B) and protein (D) bands were quantified in H460 cells. Values are expressed as mean ± SEM, n = 3 in each group. *P < 0.05, **P < 0.01 versus control group.