Table 1.
List of reagents
| Reagent | Vendor | Catalog No. |
|---|---|---|
| pAD/CMV/V5-DEST Vectors | Invitrogen | V493-20 |
| DMEM | Invitrogen | 10567-014 |
| FBS | Invitrogen | 16000-044 |
| Opti-Mem | Invitrogen | 11058-021 |
| PBS | Sigma | P3326-10UG |
| TRIzol | Invitrogen | 15596-026 |
| PureLink DNAse set | Invitrogen | 12185010 |
| PureLink RNA mini kit | Invitrogen | 12183018A |
| High-Capacity cDNA Reverse Transcription kit | Applied Biosystems | 4368814 |
| Power SYBR green Mastermix | Applied Biosystems | 4368702 |
| BCA protein assay | Pierce | 23227 |
| Transwell | Costar, Corning | 3422 |
| Amersham ECL Plus Western Blotting Detection reagents | GE Healthcare Santa Cruz Biotech | RPN2132 |
| Polyvinylidene difluoride membranes | Bio-Rad | 162-0174 |
| NuPAGE Novex 4-12% bis-Tris gels | Invitrogen | NP0322BOX |
| Cell Counting Kit-8 | Beyotime Inst Biotech | C0038 |
| Annexin V-PE Apoptosis kit | BD Biosciences | 559763 |
| IGF-1 | Sigma | I1271 |