Figure 1.

Culture and identification of three kinds of cells. A. Neural stem cells proliferate and form neurospheres. B. Neural stem cells show nestin-positive. C. Neural stem cells differentiate into neuron and show NSE positive. D. Neural stem cells differentiate into neuron and show GFAP positive. E. BMECs presented a long spindle-shape morphology. F. VIII factor related antigen immunohistochemistry stain that cytosolic and perinuclear regions were positive. G. Astrocyte culture show the long processes. H. Immunocytochemical stain show GFAP positive in cytoplasmic of astrocyte.