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. 2015 Aug 15;8(8):13136–13144.

Figure 2.

Figure 2

Expression analysis of proteins within the insulin signaling pathway after vibration exercise (VE). A, B: Western blot analysis of IRS-1 and phosphorylated IRS-1-Ser307 protein expression. C, D: Western blot analysis of the expression and phosphorylation of PI3K, AKT, and GLUT4. β-actin served as the loading control. Values are presented as mean ± SE; n = 10; For each panel: a, control group; b, high-fat diet (HFD) and streptozotocin (STZ); c, HFD and STZ with high-frequency VE; d, HFD and STZ with medium-frequency VE; e, HFD and STZ with low-frequency VE. *P < 0.05, **P < 0.01 vs. a; #P < 0.05, ##P < 0.01 vs. b.