Figure 1.
Protective effect of APα on Aβ25-35-induced cytotoxicity in cultured differentiated PC12 cells. A: PC12 cells were treated with the indicated concentrations (0-40 μM) of Aβ25-35 for 24 h; B: PC12 cells were pretreated with different concentrations of APα (10 μM) for 2 h and then incubated with Aβ25-35 (20 μM) for an additional 24 h. Viability of cells was assessed by MTT assay; C: Representative photographs of cell morphology by living cell picture (400 ×). PC12 cells treated with only Aβ25-35 can lead to cell nucleus pycnosis, nuclear fragmentation and reduce the number of cells. Application of 10 μM APα can maintain normal cell morphology and cell number. Percentage of cell viability was relative to the untreated control cells. Values represent as mean ± SD of 3 independent experiments. *P < 0.01 versus Aβ25-35-treated cells; #P > 0.05 versus control.