Table 1.

The effects of medical ozone on MDA and GSH levels as well as MPO activities in kidney tissue, plasma TNF-α and IL-1β levels that are exposed to Mtx induced nephrotoxicity. Each group was consisting of 6 rats. Groups of data were compared with an analysis of variance (ANOVA) followed by Tukey’s multiple comparison tests

	Control	Mtx	Mtx-Ozone
MDA (nmol/g tissue)	26.34±2.78	62.46±4.19**	32.57±3.26++
GSH (nmol/g tissue)	1675.24±89.24	987.62±73.69**	1852.47±153.64++
MPO (U/g tissue)	17.58±3.62	38.72±2.92***	23.45±1.97*,+++
TNF-α (pg/ml)	8.7±0.9	27.2±3.8***	10.1±2.2+++
IL-1β (pg/ml)	9.5±1.2	23.4±1.8**	11.4±1.7++

Data are mean ± sd.

^* P<0.01 compared to control group;

^** P<0.001 compared to control group;

^*** P<0.0001 compared to control group.

⁺ P<0.01 compared to Mtx group;

⁺⁺ P<0.001 compared to Mtx group;

⁺⁺⁺ P<0.0001 compared to Mtx group.