Figure 2.

CSN6 increases p27 poly-ubiquitination. (A) 293T cells were transfected with indicated expressing plasmids. MG132 was added 6 h before they were harvested. The cell lysates was then immunoprecipitated with anti-Flag and immunoblotted with anti-HA antibody. Equal amount of whole cell lysates were immunoblotted with anti-myc or Actin. (B) HCT116 cells were transfected with CSN6 or knocked down with CSN6 shRNA. The cell lysates from indicated cells were immunoprecipitated with anti-p27 and immunoblotted with anti-ubiquitin antibody. Equal amounts of cell lysates were analyzed by IB with the indicated antibodies.