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. 2015 May 6;14(14):2265–2273. doi: 10.1080/15384101.2015.1046655

Figure 3.

Figure 3.

COP1-mediated nuclear export of p27 is involved in CSN6-mediated p27 ubiquitination (A) COP1 is downregulated following the CSN6 knockdown. 293T cells were co-transfected with the indicated expression vectors. Lysates were immunoblotted with the indicated antibodies. (B) CSN6-mediated p27 downregulation is diminished by the leptomycin B. 293T cells were co-transfected with increasing myc-CSN6 expression vectors. Cells were treated with or without leptomycin B (20 ng/ml) for 6 hours before lysates were collected. Lysates were immunoblotted with the indicated antibodies. (C) Leptomycin B rescued COP1-mediated p27 downregulation. 293T cells were co-transfected with the indicated expression vectors. Cells were treated with or without leptomycin B (20 ng/ml) for 6 hours before lysates were collected. Lysates were immunoblotted with the indicated antibodies. (D) Mutation in the COP1 nuclear export signal (NES) sequence impaired the ability of COP1 to downregulate p27 expression. 293T cells were co-transfected with the indicated expression vectors. Lysates were immunoblotted with the indicated antibodies.