Table 1.

Selection-independent seamless genome editing.

Programmable Nuclease	Host Cell	Target	Template	Edits Introduced	Reference
ZFN	K562	IL2Rγ	plasmid	1 bp substitution	[14]
	CD4+ T
ZFN	K562	RSK2	ssODN	Substitution of 6 bp in and out of ZFN recognition site	[16]
ZFN	ES	SNCA	ssODN	1 bp substitution	[17]
	iPS		plasmid
TALEN	iPS	CCR5	ssODN	2 bp substitutions	[18]
CRISPR
TALEN	iPS	AKT2	ssODN	2 bp substitutions	[6]
TALEN	iPS	PHOX2B	ssODN	1 bp substitution	[19]
		PRKAG2