Table 2.
Selection-dependent seamless genome editing.
| Programmable Nuclease | Excision Method | Host Cell | Target | Edits Introduced | Reference |
|---|---|---|---|---|---|
| ZFN | Cre/loxP | ES iPS | SNCA | 1 bp substitution and loxP site insertion | [17] |
| ZFN | piggyBac | iPS | A1AT | 1 bp substitution of interest and 2 to generate TTAA site | [20] |
| CRISPR | piggyBac | iPS | HBB | 1 bp substitution and 4 bp insertion * | [15] |
| TALEN | piggyBac | iPS | HBB | 3 bp substitutions | [21] |
| TALEN | TALEN | HCT116 | Interge region (upstream of BUBR1) | 1 bp substitution | [5] |
* Two different β-thalassemia mutations (a single nucleotide substitution and a 4 bp deletion) were corrected in two different alleles. Therefore, the correction is a single-base pair edit and a 4-bp insertion in each allele.