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. 2015 Sep 7;16(9):21410–21427. doi: 10.3390/ijms160921410

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Bax (A), Bcl-2 (B) and Bax/Bcl-2 ratio (C) mRNA expression in macrophages cultured with various cadmium solutions. Macrophages were cultured with CdCl₂ solutions for 48 h. After incubation, cells were harvested by scraping and mRNA was measured by real-time PCR method. **, statistically significant as compared with 0 nM Cd-cells incubated in RPMI 1640 medium with 10% FBS and H₂O as vehicle. Experiments were conducted as six separate assays (each assay in three replicates).

Bax (A), Bcl-2 (B) and Bax/Bcl-2 ratio (C) mRNA expression in macrophages cultured with various cadmium solutions. Macrophages were cultured with CdCl₂ solutions for 48 h. After incubation, cells were harvested by scraping and mRNA was measured by real-time PCR method. **, statistically significant as compared with 0 nM Cd-cells incubated in RPMI 1640 medium with 10% FBS and H₂O as vehicle. Experiments were conducted as six separate assays (each assay in three replicates).