Table 1.
Primers and PCR amplification efficiencies.
| Gene | Primer Sequence (5ʹ–3ʹ) | Length (bp) | PCR Efficiency (%) | Correlation Coefficient (R2) | Genbank Accession No. |
|---|---|---|---|---|---|
| ACTB | F: ATTGCAGACAGGATGCAGAA R: GAAAGGGAAGCCAAGATGG |
125 | 98.5 | 0.998 | KR135165 |
| GAPDH | F: GCCCAGAACATCATCCCATCT R:CGTCATCCTCAGTGTAACCCAAG |
235 | 96.0 | 0.997 | AB094145 |
| EF-1α | F: CCACAAAGGCAGGTGAAAAGG R: ATTGGGTGAACCAAGCAGGG |
110 | 100.6 | 0.998 | KR135166 |
| UB | F: TCCAGCCTCTCCTGCCTT R: CCTTCCTTATCCTGAATCTTTGCC |
172 | 103.6 | 0.997 | KR135167 |
| TUB | F: CCACTTCCCTCTCGTCACC R: AACAGCACGCCATGTACTTT |
155 | 103.5 | 0.992 | KR135168 |
| TBP | F: AAGGAAATACGCTCGGATTG R: CTGGCTGTGAGTGAGGACAA |
136 | 100.1 | 0.997 | KR135169 |
| EIF | F: GGAATAAGGGGACGAAGACC R: GCAAACACACGCTGGGAT |
126 | 98.9 | 0.997 | KR135170 |
| 18S | F: TCCGCATCACACTCACGT R: TGGAACCCTCTCCACAGG |
165 | 100.6 | 0.997 | KR135172 |
| Vg | F: CCAGAAGACGCCACAAGAA R: CAGAAGGCATCAGCCAATC |
170 | 99.4 | 0.999 | KR135171 |
| Pcna | F: AGAGGCGGACTGAAGAGG R: TTGATGGCATCCAGCACT |
133 | 100.6 | 0.995 | KR135173 |