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. 2015 Sep 18;16(9):22621–22635. doi: 10.3390/ijms160922621

Figure 7.

Figure 7

GM-CSF, M-CSF, or LPS increased IL-10 expression in BVRA positive macrophages. (A) IL-10 mRNA expression were detected by qRT-PCR in AV-BVRA macrophages stimulated with GM-CSF; (B) IL-10 mRNA expression were detected by qRT-PCR in AV-BVRA macrophages stimulated with M-CSF; (C) IL-10 mRNA expression were detected by qRT-PCR in AV-BVRA macrophages stimulated with LPS; (D) IL-10 protein expression were detected by ELISA in AV-BVRA macrophages stimulated with GM-CSF; (E) IL-10 protein expression were detected by ELISA in AV-BVRA macrophages stimulated with M-CSF; (F) IL-10 protein expression were detected by ELISA in AV-BVRA macrophages stimulated with LPS. n = 4, *** p < 0.001, ** p < 0.01. AVBVRA: macrophages transfected with AV-BVRA; AV+GM-CSF: macrophages transfected with AV-negative control virus and stimulated with GM-CSF; AVBVRA+GM-CSF: Macrophages transfected with AV-BVRA virus and stimulated with GM-CSF; AV+M-CSF: Macrophages transfected with AV-negative control virus and stimulated with M-CSF; AVBVRA+M-CSF: Macrophages transfected with AV-BVRA virus and stimulated with M-CSF; AV+LPS: Macrophages transfected with AV-negative control virus and stimulated with LPS; AVBVRA+LPS: Macrophages transfected with AV-BVRA virus and stimulated with LPS.