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. 2015 Sep 22;16(9):22888–22903. doi: 10.3390/ijms160922888

Figure 2.

Figure 2

NlAKTIP mRNA expression in BPH. (A) Expression patterns of NlAKTIP mRNA at different developmental stages of the Tn and Rh colonies. The mRNA level of NlAKTIP was detected using RT-qPCR. The mRNA level was normalized relative to the β-actin transcription levels and the reference was the mRNA level of 1st–2nd Tn instar nymph. The results are expressed as the means ± S.E from three replicates. 1–2N: 1st–2nd instar nymph; 3–5N: 3rd–4th instar nymph; 5N: 5th instar nymph; EF: newly eclosed female; GF: gravid female, M: male; (B) NlAKTIP expression in the gravid adult female. The mRNA level of NlAKTIP was detected using RT-qPCR. The mRNA level was normalized relative to the β-actin transcription levels and the reference was the mRNA level of the Tn gravid adult female. Asterisks represent significant differences between reference Tn and other subjects (Student’s t-test, ** p < 0.01). The results are expressed as the means ± SE from three replicates.