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. 2015 Sep 22;16(9):22888–22903. doi: 10.3390/ijms160922888

Figure 3.

Figure 3

Effects of NlAKTIP RNAi. CK: female fed on D-97 (untreated diet); dsGFP: female fed on 0.5 μg/μL dsGFP control; 0.02: female fed on 0.02 μg/μL dsAKTIP; 0.1: females fed on 0.1 μg/μL dsAKTIP; 0.5: female fed on 0.5 μg/μL dsAKTIP. (A) NlAKTIP mRNA levels after feeding D-97, dsGFP or dsAKTIP. The results are expressed as the means ± SD from 20 individuals with three replications. The relative gene expression level was compared by Tukey’s test at each sampling time, and different letters of a, b, and c on the bars indicated significant differences among treatments (p < 0.05); (B) Western blot analysis of AKTIP from the different treatments; (C) Average body weights of different groups at day 7. The results are expressed as the means ± SD from at least 7 individuals. Symbols of a, b, and c indicate significant differences between each two groups (Student’s t-test, p < 0.05); (D) The effect of NlAKTIP RNAi on the adult female body size.