Table 1.

Primers used in cDNA sequencing, RT-qPCR analysis and dsRNA synthesis of the NlAKTIP.

Primer Name	Sequence (5′→3′)
cDNA cloning
3′RACE outer	TCAAACGGCAAGGCTCAT
3′RACE inner	CGTCCACAACAATCTTCTTCGC
5′RACE outer	AGGAGAAGAAGCGGAGGG
5′RACE inner	GGCATAACATAGAGCCCTGGAA
AKTIP-FL-F	ACATGGGAGACAATAACACATAACC
AKTIP-FL-R	CGACTTTAAAGCTTCTCTTGCTGTC
RT-qPCR
QAKTIP-F	TGTCTGCCAAAATGATCGAAC
QAKTIP-R	CTCCATGATAGAGCCCTT
Actin-F	TGCGTGACATCAAGGAGAAGC
Actin-R	CCATACCCAAGAAGGAAGGCT
dsRNA synthesis
dsAKTIP-F	GGATCC$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$$\underset{~}{\text{A}}$$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$$\underset{~}{\text{C}}$$\underset{~}{\text{G}}$$\underset{~}{\text{A}}$$\underset{~}{\text{C}}$$\underset{~}{\text{T}}$$\underset{~}{\text{C}}$$\underset{~}{\text{A}}$$\underset{~}{\text{C}}$$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$GGGAGAGCTCTATGTTATGCCCTCCG
dsAKTIP-R	GGATCC$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$$\underset{~}{\text{A}}$$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$$\underset{~}{\text{C}}$$\underset{~}{\text{G}}$$\underset{~}{\text{A}}$$\underset{~}{\text{C}}$$\underset{~}{\text{T}}$$\underset{~}{\text{C}}$$\underset{~}{\text{A}}$$\underset{~}{\text{C}}$$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$GGGAGAGATGCTTCTTGGTTGACTGC
dsGFP-F	GGATCC$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$$\underset{~}{\text{A}}$$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$$\underset{~}{\text{C}}$$\underset{~}{\text{G}}$$\underset{~}{\text{A}}$$\underset{~}{\text{C}}$$\underset{~}{\text{T}}$$\underset{~}{\text{C}}$$\underset{~}{\text{A}}$$\underset{~}{\text{C}}$$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$GGGATACGTGCAGGAGAGGAC
dsGFP-R	GGATCC$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$$\underset{~}{\text{A}}$$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$$\underset{~}{\text{C}}$$\underset{~}{\text{G}}$$\underset{~}{\text{A}}$$\underset{~}{\text{C}}$$\underset{~}{\text{T}}$$\underset{~}{\text{C}}$$\underset{~}{\text{A}}$$\underset{~}{\text{C}}$$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$$\underset{~}{\text{T}}$$\underset{~}{\text{A}}$GGGCAGATTGTGTGGACAGG

Underlined sequences represent for protective bases, and wavelined sequences represent for T7 promoter.