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. 2015 Sep 22;16(9):22904–22926. doi: 10.3390/ijms160922904

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Validation of the gene expression profile by qRT-PCR. Each dot represents an individual goose in one of the two groups (one group is two-week-old goslings; the other group is adult geese). The mRNA level of each spleen sample was quantified by qRT-PCR in triplicate for each gene. GAPDH was amplified as an internal control. The chart was constructed in GraphPad Prism 5. The relative expression levels were compared using multiple t-tests. Data are represented as the mean ± SEM (n = 3). For gene symbols representing different genes, refer to Table 4. * indicates significant differences (p ≤ 0.05), ** indicates extremely significant differences (p ≤ 0.01).