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Variation of Cq values for P. pachyrhizi candidate reference genes between samples (vertical axis: Cq). (a) Average and standard deviation of Cq values for every sample; (b–d) box plots representing the range of Cq values; (b) in vitro samples; (c) in planta samples; (d) all samples. For better representation of RNA abundance the Cq values were corrected for primer efficiency: Cqcorr = lg2 ((2 × e)Cq).
