Table 3.
Primers used in qRT-PCR for P. pachyrhizi genes.
| Name | Gene | Sequence | Reference | Amplicon (bp) | Efficiency (%) |
|---|---|---|---|---|---|
| ActinDis 1 f (KES 1461 fw) | Act | acagtttcaccacaaccgcc | [18] | 144 | 93 |
| ActinDis 1 r (KES 1462 rv) | tgaccgtcgggaagttcg | ||||
| qPpGI363fw | ASUB | tgtcccaaccctttgctgtt | this study | 146 | 86 |
| qPpGI363rv | ggcaccgaccatgtaaaacg | ||||
| AtubDis 1 f (KES 1463 fw) | aTub | ctgcgaacaactatgctcgtc | [18] | 116 | 90 |
| AtubDis 1 r (KES 1464 fw) | cacgaagaagccttggagtcc | ||||
| CytB 1 f (KES 1688 fw) | CytB | tcaagacgcatccaattctaggtc | [28] | 138 | 85 |
| CytB 1 r (KES 1689 rv) | gtgttacacccgtgataatctgaatgat | ||||
| Elf1a 1 f | Elf1a | gtgagcgtggtatcaccatc | this study | 143 | 92 |
| Elf1a 1 r | cagaatggcgcaatcagc | ||||
| q00583fw | Elf3 | aatgcgtggtctctctggtg | this study | 89 | 95 |
| q00583rv | gctcgtccaagatcaccaca | ||||
| GAPDH 1 f (KES 1465 fw) | GAPDH | ggtatggctttccgagttcca | [18] | 157 | 90 |
| GAPDH 1 r (KES 1466 rv) | tcagttgataccaaatcatcctcag | ||||
| q00239fw | PDH | gcggaaggaaaggataagggg | this study | 136 | 95 |
| q00239rv | tccgatccttagtctggcct | ||||
| q02726fw | PDK | acctcccgttcagctagtct | this study | 138 | 94 |
| q02726rv | aattcatcagagtcggcccc | ||||
| RibPro 3 f | RPS9 | gtgaatgggagaccaatctcag | this study | 128 | 94 |
| RibPro 3 r | ttgcctcctccatgagtcag | ||||
| q00682fw | RPS11 | ggactgggcttcaagactcc | this study | 97 | 86 |
| q00682rv | gaatcctgcccctgatcgag | ||||
| q00153fw | RPS14 | agttgctcgagtgactggtg | this study | 86 | 89 |
| q00153rv | catcttgggcagccaacatg | ||||
| q00241fw | SDH | caatcgcctgaggaccgtaa | this study | 80 | 88 |
| q00241rv | ctggggcaacttgtagagca | ||||
| Ubc 1 f | Ubc | cggaccagtacccttacaaatc | this study | 128 | 93 |
| Ubc 1 r | atcaaacatcggcgaccag | ||||
| UbcE2 3 f | UbcE2 | gtcgaactgtgacgagtttg | this study | 117 | 89 |
| UbcE2 3 r | acggccttagtcttcgatg |