Figure 1.

Schematics of the simplified overview of deep mutational scanning: (a) generation of the initial protein variant library for target protein sequences; (b) screening for protein variants with desired properties; and (c) sequencing and quantification of the mutations under different selection pressures. The asterisks indicate mutations at a specific site and the stacked asterisks indicate enrichment of mutations in specific sites after quantification. For example, mutation counts at different sites are shown with * positions carrying a mutation, ** positions carrying two mutations, and **** positions carrying four mutations.