Table 1.

Recent studies using deep mutational scanning.

Mutation Generation Method 1	Variant Library	Sequencing Method 2	Target Protein 3	Reference
ORM	Phage display	Solexa/PE	PSD95pdz3	[10]
ORM	Bacterial two-hybrid	Illumina/PE	hYAP65	[22]
ORM	Yeast two-hybrid	Illumina/SE	BRCA1	[27]
PRM	Plasmid	Illumina/SE	EcFbFP	[21]
SM	Yeast display	Illumina/PE	HB80.3	[24]
ORM	Plasmid	Illumina/PE	APH(3′)II	[28]
SM	Plasmid	Illumina/PE	Bgl3	[23]
SM	Plasmid	454	CcdB	[26]
ORM	Plasmid	Illumina/PE	Pab1	[29]
ORM	Mammalian display vectors	454	IgG	[30]
ORM	Ribosome display	454	CDR loops of Fab	[47]
ORM	Phage display	Illumina/PE	hYAP65	[48]

¹ ORM: Oligonucleotide-directed random mutagenesis; PRM: PCR-based random mutagenesis; SM: Saturated mutagenesis; ² PE: paired-end; SE: single-end; ³ PDZ domain: post synaptic density protein; hYAP65: human Yes-associated protein 65; BRCA1: breast cancer 1 (early onset); EcFbFP: Escherichia coli flavin mononucleotide binding fluorescent protein; HB80.3: HB80.3 (designed high affinity binding protein); APH(3′)II: Tn5 transposon derived aminoglycoside-3′-phosphotransferase-II; Bgl3: β-glucosidase; CcdB: bacterial toxin protein CcdB; Pab1: poly(A)-binding protein; IgG: immunoglobulin G; CDR: complementary determining region; Fab: fragment antigen-binding region.