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. 2015 Jan 15;14(4):612–620. doi: 10.4161/15384101.2014.992191

Figure 1.

Figure 1.

Hoxa5 is predominantly expressed in haematopoietic stem cells. (A) RNA-seq showed higher expression level of Hoxa5 in HSCs. Murine HSCs are defined as Lin (CD2, CD3, CD4, CD8, Mac1, Gr1, Ter119, B220)-, CD48-, Sca1+, cKit+, and CD150+. MPPs are defined as Lin (CD2, CD3, CD4, CD8, Mac1, Gr1, Ter119, B220)-, CD48-, Sca1+, cKit+, and CD150-. Total RNA of 10,000 sorted HSCs and MPPs was extracted using RNeasy micro kit. Two nanograms of HSC and MPP RNA were used as the RNA input for RNA-seq sample preparation. Three independent experiments are shown. (B) Real time PCR confirmed the expression pattern of Hoxa5 in HSCs and MPPs. Two nangrams of RNA from sorted HSCs and MPPs were linearly amplified. Ten nanograms of cDNA were used as the input for q-RT-PCR. The relative fold increase was calculated by a value of 2^(-ΔΔCt). The value of MPP was normalized to 1 for comparison. Error bars indicate standard error. (**) P < 0.01.