Figure 3.

HELP domain and N-terminal WD40 domains in EML4 are required for mitotic progression. (A) Schematic representation of EML4 deletion mutants. (B) Cells that constitutively expressed GFP-tagged EML4 deletion mutants were lysed, and the expression of each mutant was examined by immunoblot. (C) Each GFP-EML4 cell line was transfected with EML4 siRNA#2, and the cells were immunostained for GFP and tubulin 72 h later (Scale bar = 5 μm). (D) The graph shows the percentage of metaphase cells with the indicated defect. Three independent experiments were performed, and more than 150 cells in total were evaluated (mean ± SEM, ***P < 0.01, n.s; not significant compared to Ctrl siRNA).