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. 2015 Mar 19;14(10):1529–1539. doi: 10.1080/15384101.2015.1026514

Figure 5.

Figure 5.

WD40 domains of EML4 and the C-terminus of NUDC are required for the interaction between these proteins. (A) Schematic representation of EML4 deletion mutants. (B) 293T cells were transfected with a plasmid encoding each gene, and the cells were lysed 24 h later. GFP-tagged proteins were immunoprecipitated with anti-GFP antibody and immunoblotted for Flag and GFP. (C) The interaction between Flag-NUDC and GFP-tagged EML4 deletion mutants was examined. (D) Schematic representation of NUDC deletion mutants. (E) The interaction between Flag-EML4 and GFP-tagged NUDC deletion mutants was examined.