Figure 6.

EML4 is required for NUDC localization to the mitotic spindle. (A) HeLa cells were transfected with the indicated siRNAs. Seventy-two hours later, the cells were lysed, and the expression of the indicated protein was determined by immunoblot. (B) HeLa cells transfected with control or NUDC siRNAs were lysed and level of EML4 mRNA was determined by real time PCR analysis. The graph show the relative level of EML4 mRNA normalized to the level of GAPDH mRNA. (C). Cells were transfected with siRNAs, and the cells were immunostained for NUDC or EML4 72 h later (Scale bar = 5 μm). (D) Cells that constitutively expressed each GFP-tagged EML4 deletion mutant were transfected with EML4 siRNA#2. After 72 h, the cells were fixed and immunostained for NUDC and GFP (Scale bar = 5 μm).