. 2015 Mar 27;128(12):895–904. doi: 10.1042/CS20140705

Table 1. Peak exercise parameters, ³¹P-MRS examination on calf evaluating in vivo mitochondrial function and assessment of respiratory chain complex activities in skeletal muscle homogenates.

BPM, beats per min; DCPIP, 2,6-dichlorophenol-indophenol; n/a, not available; t1/2 PCR (s), half time for PCR recovery from end exercise to baseline concentrations; VO₂, oxygen uptake. Enzyme activities are expressed as nmol of NADH oxidized/min per unit of citrate synthase (CS) for CI, nmol of DCPIP reduced/min per unit of CS for CII (succinate:ubiquinone-1 reductase) and the apparent first-order rate constant/s per unit of CS for CIII and CIV (×10³).

Parameter	Patient 1	Patient 2	Reference data (means ± S.D.)
CPET
Peak exercise data	Absolute value (% of predicted)	Absolute value (% of predicted)	n/a
Heart rate (BPM)	172 (89)	180 (92)	n/a
VO₂ (ml/min)	0.575 (27)	0.638 (22)	n/a
VO₂ (ml/min per kg)	8 (27)	14 (22)	n/a
VO₂ (% of predicted VO₂)	27	22	n/a
Power (W)	35 (21)	53 (27)	n/a
AT
ATVO₂ (ml/min per kg)	6	10	n/a
ATVO₂ (% of predicted peak VO₂)	20	16	n/a
ATVO₂ (% of recorded peak VO₂)	75	74	n/a

³¹P-MRS			[14]
End exercise data
t1/2 PCr	52.5	n/a	29.2 ± 3.1
t1/2 ADP	31.4	n/a	20 ± 1

Respiratory chain complex activities	Biopsy 1 Biopsy 2	Biopsy	Controls (n=25)
CI/CS	0.018 0.009	0.001	0.104 ± 0.036
CII/CS	0.092 0.127	0.070	0.145 ± 0.047
CIII/CS	0.673 1.790	0.634	0.554 ± 0.345
CIV/CS	0.834 1.394	1.216	1.124 ± 0.511

Table 1. Peak exercise parameters, 31P-MRS examination on calf evaluating in vivo mitochondrial function and assessment of respiratory chain complex activities in skeletal muscle homogenates.

Table 1. Peak exercise parameters, ³¹P-MRS examination on calf evaluating in vivo mitochondrial function and assessment of respiratory chain complex activities in skeletal muscle homogenates.