Figure 7. A3BCD2 Tyr³¹³ mutation enhances the substrate specificity on mC.

(A) Gel image showing the C deamination activity of A3BCD2 mutants. Each protein (0.5 μM and 2 μM) was incubated with 600 nM 30 nt ssDNA substrates at 37°C for 2 h. (B) Gel image showing the mC deamination activity of A3BCD2 mutants. Each protein (0.5 μM and 2 μM) was incubated with 600 nM 30 nt ssDNA substrates at 37°C for 2 h. (C) Dose-response of A3BCD2 WT and Y313F for C and mC deamination. (D) Comparison of the mC selectivity factor for A3BCD2 WT and Y313F. The data were calculated from Table 1. Error bars represent S.D. from the mean for three independent experiments.