Table 1. Comparison of the deaminase activity and mC selectivity factor of various A3BCD2 mutants.
A3BCD2 | A3A | Mt0 | M3 | M4 | M3M4 | Y313F | |
---|---|---|---|---|---|---|---|
C deamination activity* (nM product/μM enzyme) | 506.4±30.1 | 401900±20230 | 3733±230.5 | 498.7±65.7 | 3605±449.0 | 6943±124.8 | 55.32±7.7 |
mC deamination activity* (nM product/μM enzyme) | 10.22±0.4 | 51030±1480 | 566.8±72.5 | 41.81±2.2 | 353.4±27.8 | 1309±70.8 | 7.572±0.5 |
mC selectivity factor† [(mC/C activity) × 100] | 2.01 | 12.70 | 15.18 | 8.38 | 9.80 | 18.85 | 13.69 |
*Deaminase activity (nM product/μM enzyme) for the C and mC deamination was calculated from the initial linear range of dose-response assays for each protein construct (Supplementary Figures S3B, S3C, S6B, S7C, S7D and S8A). S.D. was estimated from data collected in three independent experiments.
†The mC selectivity factor was calculated as [mC/C activity × 100], where the deaminase activity for C and mC was used in this calculation.