Figure 3.

Various single-gene-deletion mutations (each eliminating an enzyme involved in POA transport to the ER or in POA incorporation into PL and/or NL within the ER) elicit differential effects on the susceptibility of yeast to POA-induced liponecrotic PCD. WT and mutant cells were recovered at days 1, 2 and 4 of culturing in YP medium initially containing 0.2% glucose as carbon source. Cell survival was assessed by measuring the clonogenicity of cells after 2 h of treatment with various concentrations of exogenous POA. Data for the effect of the dga1Δ mutation on the susceptibility of yeast to POA-induced liponecrosis are from a different series of experiments than the ones reported in our recent publication [1]. Data are presented as means ± SEM (n = 12–19).